Regulation of quinolate phosphoribosyl transferase expression
AssigneeNorth Carolina State University
InventorsMark A. Conkling, Wen Song, Nandini Mendu
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Abstract
DNA encoding a plant quinolate phosphoribosyl transferase (QPRTase) enzyme, and constructs comprising such DNA are provided. Methods of altering quinolate phosphoribosyl transferase expression are provided.
Claims28
A method of making a transgenic tobacco plant cell having increased quinolate phosphoribosyl transferase (QPRTase) expression, said method comprising:
- transforming a tobacco plant cell with an exogenous nucleic acid construct, which construct comprises, in the 5′ to 3′ direction, a promoter operable in a tobacco plant cell and a nucleotide sequence encoding a quinolate phosphoribosyl transferase comprising the amino acid sequence of SEQ ID NO:2, wherein said nucleotide sequence is operably associated with said promoter to produce transformed tobacco plant cells, said tobacco plant cells having increased expression of QPRTase compared to an untransformed tobacco plant cell.
A transgenic plant of the genus Nicotiana having increased quinolate phosphoribosyl transferase (QPRTase) expression relative to a non-transformed control plant, said transgenic plant comprising transgenic plant cells containing:
- an exogenous nucleic acid construct comprising, in the 5′ to 3′ direction, a promoter operable in said plant cell and a nucleotide sequence encoding a plant quinolate phosphoribosyl transferase comprising the amino acid sequence of SEQ ID NO:2, said nucleotide sequence operably associated with said promoter;
- said plant exhibiting increased QPRTase expression compared to a non-transformed control plant.
A method for increasing expression of a quinolate phosphoribosyl transferase gene in a tobacco plant cell, said method comprising:
- growing a tobacco plant cell transformed to contain exogenous nucleic acid, wherein said exogenous nucleic acid encodes a quinolate phosphoribosyl transferase comprising the amino acid sequence of SEQ ID NO:2.
A method of producing a tobacco plant having an increased amount of nicotine in leaves of said tobacco plant, said method comprising:
- growing a tobacco plant, or progeny plants thereof, wherein said plant comprises cells containing a nucleic acid construct comprising a transcriptional initiation region functional in said plant and an exogenous nucleotide sequence operably joined to said transcriptional initiation region, wherein said nucleotide sequence encodes quinolate phosphoribosyl transferase comprising the amino acid sequence of SEQ ID NO:2.
Dependencies are inferred from the claim text.