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US 7,408,096 B2Granted
Expression of Cry3B insecticidal protein in plants
AssigneeMonsanto Technology LLC
InventorCharles P. Romano
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AssigneeMonsanto Technology LLC
InventorCharles P. Romano
Estimating the term…
The present invention discloses methods and compositions comprising a novel expression cassette providing significantly improved levels of accumulation of Coleopteran inhibitory Cry3B variant protein when expressed in maize plants. The preferred embodiments of the invention provide at least up to ten fold higher levels of insect controlling protein relative to the highest levels obtained using prior compositions. In particular, transgenic maize expressing higher levels of a protein designed to exhibit increased toxicity toward Coleopteran pests deliver superior levels of insect protection and are less likely to sponsor development of populations of target insects that are resistant to the insecticidally active protein.
An expression cassette comprising the nucleotide sequence as set forth in SEQ ID NO:19 from nucleotide 14 through nucleotide 3020.
A method of producing a transformed cell, said method comprising introducing into a cell an expression cassette for expression of an insecticidal protein, wherein said expression cassette comprises the nucleotide sequence as set forth in SEQ ID NO:19 from nucleotide 14 through nucleotide 3020, and wherein said cell is a plant cell or a microbial cell.
A method of producing an insect resistant maize plant, said method comprising introducing into a maize plant cell the expression cassette as set forth in SEQ ID NO:19 for expression of an insecticidal protein, selecting a transformed maize plant cell, and regenerating a maize plant from said transformed maize plant cell, wherein said maize plant comprises said expression cassette.
A plant comprising the expression cassette as set forth in SEQ ID NO:19 from nucleotide 14 through nucleotide 3020.
A method of controlling Coleopteran insect infestation in a field of crop plants, said method comprising providing to a Coleopteran insect a transgenic plant on which said Coleopteran insect feeds, said transgenic plant expressing the insecticidal protein encoded by the polynucleotide sequence as set forth in SEQ ID NO:19 from nucleotide 14 through nucleotide 3020.
A method of controlling Coleopteran insect infestation of a plant, said method comprising providing to a Coleopteran insect a transgenic plant on which said Coleopteran insect feeds, said transgenic plant expressing the insecticidal protein encoded by the polynucleotide sequence as set forth in SEQ ID NO:19 from nucleotide 14 through nucleotide 3020.
A vector comprising a polynucleotide sequence encoding the peptide as set forth in SEQ ID NO:20.
Dependencies are inferred from the claim text.