Production of secreted polypeptides
AssigneeGenencor International, Inc.
InventorMaria R. Diaz-Torres
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Abstract
Described herein are methods and compositions for the production and secretion of polypeptides. Included herein is the use of interrupting peptide transport activity for an increase in polypeptide production and/or secretion.
Claims3
An isolated Bacillus cell comprising a peptide transport operon, wherein said operon is a Bacillus dciA operon or opp operon, and wherein said operon has been mutated by making inactivating deletions or insertions in a gene in the operon such that said cell has increased polypeptide secretion.
An isolated Escherichia coli cell comprising a peptide transport operon, wherein said operon is an Escherichia coli dpp operon or opp operon, and wherein said operon has been mutated by making inactivating deletions or insertions in a gene in the operon such that said cell has increased polypeptide secretion.
Dependencies are inferred from the claim text.