US 7,407,782 B2 · Nucleic acid detection compositions

Abstract

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Claims29

Dependent claims

A composition for detecting a target nucleic acid, comprising:

a) a purified thermostable FEN-1 endonuclease, active at 55° C. or above;
b) an oligonucleotide probe, said oligonucleotide probe configured to participate in a FEN-1 endonuclease cleavage structure in the presence of said target nucleic molecule.

Dependencies are inferred from the claim text.

Facts

Application

11/103,943Filed 12 Apr 2005 · continuation

Priority

24 Jan 1996

Published

US 2005/0181435 A118 Aug 2005

Granted

5 Aug 2008

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12N 9/22
Acting on ester bonds: ribonucleases [RNase]; Deoxyribonucleases [DNase]
C12Q 1/6823
Characterised by the detection means: release of bound markers
C12Q 1/6827
Hybridisation assays: for detection of mutation or polymorphism
C12Q 1/683
For detection of mutation or polymorphism: involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Family

Application08/599,491Parent of this continuation-in-part · filed 24 Jan 1996

Application08/682,853Parent of this continuation-in-part · filed 12 Jul 1996

Application08/756,386Parent of this division · filed 26 Nov 1996

Application09/350,309Parent of this continuation · filed 9 Jul 1999

Application09/982,667Parent of this continuation · filed 18 Oct 2001

US 2005/0181435 A1Pre-grant publication · published 18 Aug 2005

US 7,011,944 B2Parent of this continuation

US 6,348,314 B1Parent of this continuation

US 5,985,557 AParent of this division

Application09/350,309Parent of this division

US 6,001,567 AParent of this continuation-in-part

Application08/756,386Parent of this continuation-in-part

US 5,846,717 AParent of this continuation-in-part

US 2003/0096245 A1Published application

US 7,407,782 B2This patent

From the US filing history. Foreign family members aren’t listed.

Nucleic acid detection compositions

Life of this patent

Nucleic acid detection compositions

Life of this patent

Abstract

Claims29