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US 7,407,779 B2Granted
Modification of solid 3-sn-phosphoglycerides
AssigneeBioMolecular Products, Inc.
InventorsDavid W. Yesair, Walter A. Shaw, Stephen W. Burgess, Robert Travis McKee
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Abstract
Methods for hydrolyzing solid ungranulated lysophosphatidylcholine with phospholipase A2 are provided. Also disclosed are methods for making a lipid matrix of lysophosphatidylcholine, monoglyceride and fatty acid, and lipid matrices of particular structure.
Claims23
A method for modifying ungranulated/solid matrix 3-sn-phosphoglyceride molecules, comprising
- forming a reaction mixture by contacting ungranulated/solid matrix 3-sn-phosphoglyceride molecules with an amount of phospholipase A2 sufficient to modify an ester bond of the 3-sn-phosphoglyceride molecules,
- adding to the reaction mixture one or more fatty acids and one or more agents selected from the group consisting of monoglyceride;
- diglyceride;
- polyglycerol fatty acid ester;
- sucrose fatty acid ester;
- sorbitan fatty acid ester;
- glycerol and other alcohol functional groups including serine and ethanolamine;
- and solvents;
- and
- incubating the reaction mixture to hydrolyze the 2-acyl bond,
- wherein the ungranulated/solid matrix 3-sn-phosphoglyceride molecules are hydrolyzed with >99% efficiency and wherein the hydrolysis of the ungranulated/solid matrix 3-sn-phosphogglyceride molecules is complete within 7 hours.
8 more elements
A method for making lysophosphoglyceride comprising:
- contacting a mixture of ungranulated/solid matrix 3-sn-phosphoglyceride, one or more fatty acids and an agent selected from the group consisting of monoglyceride;
- diglyceride;
- polyglycerol fatty acid ester;
- sucrose fatty acid ester;
- sorbitan fatty acid ester;
- glycerol and other alcohol functional groups including serine and ethanolamine;
- and solvents, with phospholipase A2 to form a reaction mixture wherein the ungradulated/solid matrix 3-sn-phosphoglyceride molecules are hydrolyzed with >99% efficiency, and wherein the hydrolysis of the ungradulated/solid matrix 3-sn-phosphoglyceride molecules is complete within 7 hours, and
- recovering lysophosphoglyceride formed in the reaction mixture.
5 more elements
A method for making a composition containing lysophosphoglyceride, monoglyceride and fatty acid, comprising:
- contacting a reaction mixture of ungranulated/solid matrix 3-sn-phosphoglyceride, one or more fatty acids and monoglyceride with phospholipase A2 wherein the ungradulated/solid matrix 3-sn-phosphoglyceride molecules are hydrolized with >99% efficiency, and wherein the hydrolysis of the ungradulated/solid matrix 3-sn-pholphoglyceride molecules is complete within 7 hours, and
- recovering a lipid complex containing lysophosphoglyceride, monoglyceride and fatty acid, wherein the molar ratio of lysophosphoglyceride:the sum of monoglyceride and fatty acid in the recovered lipid complex composition is between 1:3 and 1:12.
Dependencies are inferred from the claim text.