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AssigneeApplera Corporation
InventorsKathleen M. Perry, Robert E. Ruhfel, Sandra L. Spurgeon, Peter Ma
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A dry composition comprising a nucleotide linked to a fluorescent dye is provided. A method of making a dry composition comprising the step of drying a solution comprising a nucleotide linked to a fluorescent dye is provided. A method is provided for performing a reaction on a nucleic acid substrate comprising: (a) providing a vessel containing a dried composition comprising a nucleotide linked to a fluorescent dye, and (b) adding to the vessel an aqueous solution and the nucleic acid substrate. A kit is provided for determining the sequence of a nucleic acid, the kit comprising a dry composition comprising a nucleotide linked to a fluorescent dye.
A dry composition comprising a mononucleotide linked to a fluorescent dye, wherein the composition comprises a thermostable nucleic acid polymerase, wherein the thermostable nucleic acid polymerase is Taq DNA polymerase, wherein the dry composition is substantially free of glycerol, and wherein the ratio of glycerol to Taq DNA polymerase is less than 1 ug of glycerol per unit of Taq DNA polymerase.
Dependencies are inferred from the claim text.