Method of using sol-gel coatings for on-line preconcentration of amino acids in capillary electrophoresis

A method of preparing sol solution coated capillary having an inner surface, comprising the steps of:

• filling a fused silica capillary with sol solution, wherein the sol solution comprises sol-gel precursors, a deactivating reagent and a sol-gel catalyst;
• allowing the sol solution in the capillary to form a stationary phase, wherein a first portion of the stationary phase forms a thin layer, chemically bonded to the inner surface of the capillary, and a second portion of the stationary phase forms a residual solution;
• expelling the residual solution;
• and
• heating the capillary in an oven within a temperature range of 250° C. to 300° C., thereby forming a prepared column for sample preconcentration in capillary electrophoresis.

A method of preparing sol solution coated capillary having an inner surface, comprising the steps of:

• filling a fused silica capillary with sol solution, wherein the sol solution comprises a sol-gel precursor, a deactivating reagent and a sol-gel catalyst;
• allowing the sol solution in the capillary to form a stationary phase, wherein a first portion of the stationary phase forms a thin layer, chemically bonded to the inner surface of the capillary, and a second portion of the stationary phase is a residual solution;
• expelling the residual solution under gas pressure;
• and
• heating the capillary in an oven within a temperature range of 250° C. to 300° C., thereby forming a prepared column for sample preconcentration in capillary electrophoresis.