CRISP polypeptides as contraceptives and inhibitors of sperm capacitation
AssigneeRegents of the University of Minnesota
InventorsDavid W. Hamilton, Kenneth P. Roberts, Kathy M. Ensrud
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Abstract
Included in the present invention are methods of inhibiting sperm capacitation, inhibiting the phosphorylation of a protein at tyrosine residues, inhibiting an acrosomal reaction, and inhibiting fertilization of an egg by sperm with the administration of a CRISP polypeptide.
Claims3
A method of inhibiting sperm capacitation comprising contacting sperm that have not undergone capacitation with a cysteine-rich secretory protein (CRISP) polypeptide under capacitation conditions, wherein the CRISP polypeptide has at least 95% sequence identity to human CRISP-1 (SEQ ID NO:
- 1) and the CRISP polypeptide inhibits tyrosine phosphorviation of sperm proteins.
A method of inhibiting sperm capacitation comprising contacting sperm that have not undergone capacitation with a cysteine-rich secretory protein (CRISP) polypeptide under capacitating conditions, wherein the CRISP polypeptide is human CRISP-1 (SEQ ID NO:1).
A method of inhibiting sperm capacitation comprising contacting sperm with the human CRISP-1 polypeptide SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.