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US 7,341,848 B2Granted
Isomaltose synthase-knockout microorganism belonging eumycota
AssigneeAmano Enzyme Inc.
InventorsKanako Suzuki, Norihiro Tsukagoshi
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AssigneeAmano Enzyme Inc.
InventorsKanako Suzuki, Norihiro Tsukagoshi
Estimating the term…
It is intended to provide a host microorganism whereby the expression of a gene encoding a target protein can be effectively elevated in the case of transferring the gene. It is also intended to provide a transformant whereby a target protein can be produced at a high efficiency. It is further intended to provide a production process whereby a target protein can be produced at a high productivity. A microorganism which belongs to Eumycota and lacks the major isomaltose synthase. A gene encoding a target protein is transferred into the microorganism to give a transformant. Then the transformant is cultured under conditions allowing the expression of the transferred gene to thereby produce the protein.
A transformant obtained by introducing a foreign gene into a microorganism which belongs to Aspergillus which lacks an α-glucosidase B gene, wherein the foreign gene comprises a structural gene and a promoter promoting a transription of the structural gene, the promoter is a promoter whose transcription activity is induced by isomaltose, and the promoter is a promoter of α-amylase gene, glucoamylase gene, or α-gloucosidase gene of Aspergillus.
A transformant obtained by introducing a foreign gene into Aspergillus nidulans which lacks an α-glucosidase B gene, wherein the foreign gene comprises a structural gene and a promoter promoting a transcription of the structural gene, the promoter is a promoter whose transcription activity is induced by isomaltose, and the promoter is a promoter of α-amylase gene, glucoamylase gene, or α-glucosidase gene of Aspergillus.
A transformant obtained by introducing a foreign gene into a microorganism which belongs to Aspergillus which lacks an α-glucosidase B gene, wherein the foreign gene comprises a structural gene and a modified promoter obtained by inserting a first DNA fragment containing CCAATNNNNNN (first base sequence:
Dependencies are inferred from the claim text.