Decaffeinating microorganism and process of bio-decaffeination of caffeine containing solutions

A simple, safe, and efficient process for the complete bio-decaffeination of caffeine-containing solutions using strain Pseudomonas alcaligenes CFR 1708, said process comprising steps of:

• a. culturing the strain in caffeine agar plates,
• b. transferring actively growing culture into nutrient broth containing 0.1–0.5 g/L caffeine,
• c. incubating culture of step (b) at about 28–30° C. for about 20–24 hrs under agitation of between about 50–200 rpm to obtain pre-inoculum,
• d. transferring the pre-inoculum to nutrient broth comprising about 0.5–2 g/L caffeine,
• e. incubating the pre-inoculum at about 28–30° C. for about 20–24 hours under agitation between about 50–200 rpm to accumulate biomass,
• f. harvesting the biomass by centrifugation for about 10–20 mm at about 10,000–16,000 g at temperature ranging between about 0–4° C. to obtain pellet,
• g. inducing the pellet for caffeine degradation by aseptically transferring the pellet into sterilized caffeine liquid medium of pH about 5–9,
• h. incubating the induced pellet at about 25–35° C. under agitation between about 50–200 rotations per minute for time duration of about 36–60 hours, and
• i. decaffeinating caffeine-containing solution by incubating with the pellet of step (h).