Salmonella typhimurium-infected Caenorhabditis elegans for identifying inhibitors of infection
AssigneeThe General Hospital Corporation
InventorsFrederick M. Ausubel, Alejandro Aballay, Peter S. Yorgey
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Abstract
Disclosed are screening methods for identifying the interplay between environmental and host signals (e.g., host-dependent or host-independent signals) and physiological pathogenic pathways that control or regulate genes responsible for establishing a persistent infection, as in the colonization of the gut of the nematode.
Claims15
An isolated Caenorhabditis elegans nematode infected with an isolated Salmonella typhimurium bacterium.
A method of screening for a compound that inhibits a S. typhimurium bacterium in a C. elegans nematode, comprising the steps of:
- (a) providing a C. elegans nematode infected with a S. typhimurium bacterium;
- (b) contacting said infected C. elegans nematode with a test compound;
- and
- (c) determining whether the test compound inhibits said S. typhimurium bacterium in said infected C. elegans nematode.
A method of screening for a compound that inhibits S. typhimurium bacterial infection in a C. elegans nematode, comprising the steps of:
- (a) providing a C. elegans nematode infected with a S. typhimurium bacterium expressing a detectable marker;
- (b) contacting said infected C. elegans nematode with a test compound;
- and
- (c) determining whether said S. typhimurium bacterium infects said C. elegans nematode by measuring the level of detectable marker in said nematode, where a decrease of the marker in said C. elegans nematode indicates that said test compound inhibits a S. typhimurium bacterial infection in the nematode.
Dependencies are inferred from the claim text.