Nucleic acid molecules encoding osteogenic proteins
AssigneeStryker Biotech
InventorsHermann Oppermann, Thangavel Kuberasampath, David C. Rueger, Engin Ozkaynak
Life of this patent
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Abstract
Disclosed are 1) osteogenic devices comprising a matrix containing osteogenic protein and methods of inducing endochondral bone growth in mammals using the devices; 2) amino acid sequence data, amino acid composition, solubility properties, structural features, homologies and various other data characterizing osteogenic proteins, 3) methods of producing osteogenic proteins using recombinant DNA technology, and 4) osteogenically and chondrogenically active synthetic protein constructs.
Claims9
A recombinant nucleotide sequence consisting of:
- (a) CTGTATGTCAGCTTCCGAGACCTGGGCTGGCAGGACTGGATCATCGCGCCTG AAGGCTACGCGCGCTACTACTGTGAGGGGGAGTGTGCCTTCCCTCTGAACTC CTACATGAACGCCACCAACCACGCCATCGTGCAGACGCTGGTCCACTTCATC AACCCGGAAACGGTGCCCAAGCCCTGCTGTGCGCCCACGCAGCTCAATGCCA TCTCCGTCCTCTACTTCGATGACAGCTCCAACGTCATCCTGAAGAAATACAGA AACATGGTGGTCCGGGCCTGTGGCTGCCACTAGCTCCT (nucleotides 16–314 of SEQ ID NO:
- 42), or
- (b) a sequence encoding an amino acid sequence:
- LYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTLV HFINPETVPKPCCAPTQLNAISVLYFDDSSNVILKKYRNMVVRACGCH (SEQ ID NO:
- 39), or a conservative amino acid variant thereof,
- wherein said conservative amino acid variant encodes a protein competent to induce bone and cartilage in a mammal.
A recombinant nucleotide sequence consisting of:
- TGTAAGAAGCACGAGCTGTATGTCAGCTTCCGAGACCTGGGCTGGCAGGACT GGATCATCGCGCCTGAAGGCTACGCGCGCTACTACTGTGAGGGGGAGTGTGC CTTCCCTCTGAACTCCTACATGAACGCCACCAACCACGCCATCGTGCAGACG CTGGTCCACTTCATCAACCCGGAAACGGTGCCCAAGCCCTGCTGTGCGCCCA CGCAGCTCAATGCCATCTCCGTCCTCTACTTCGATGACAGCTCCAACGTCATC CTGAAGAAATACAGAAACATGGTGGTCCGGGCCTGTGGCTGCCACTAGCTCC T (SEQ ID NO:
- 42), or
- a sequence encoding an amino acid sequence:
- CKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTL VHFINPETVPKPCCAPTQLNAISVLYFDDSSNVILKKYRNMVVRACGCH (amino acids 6–107 of SEQ ID NO:
- 9), or a conservative amino acid variant thereof,
- wherein said conservative amino acid variant encodes a protein competent to induce bone and cartilage in a mammal.
Dependencies are inferred from the claim text.