Highly transformable bacterial cells and methods for producing the same
AssigneeStratagene California
InventorsAlan Lewis Greener, Bruce Douglas Jerpseth
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Abstract
The invention provided herein includes novel gram negative bacteria cells containing the Hte mutation. Other aspects of the invention include methods for rendering gram negative bacterial cells bearing the Hte region, such as E. coli cells competent for DNA transformation using any of a variety of competency inducing procedures. The competent cells of the subject invention may be frozen so as to provide for prolonged storage.
Claims2
An isolated strain of gram negative bacteria comprising an Hte mutation included in the strain deposited as ATCC No. 55962 and having more efficient transformation with foreign plasmids than gram negative bacteria that lack said Hte mutation.
Dependencies are inferred from the claim text.