Preparing membrane receptors from extracellular baciloviruses
AssigneesCentre National De La Recherche Scientifique, Valorisation-Recherche, Limited Partnership
InventorsMichel Bouvier, Thomas Loisel, Stefano Marullo, Pierre Boulanger, Arthur Donny Strosberg
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Abstract
Method of producing a recombinant membrane receptor protein by introducing a baculovirus expression vector encoding the membrane receptor protein into a host cell, culturing the resultant host cell for a sufficient time to permit expression of the membrane receptor protein and baculovirus viral particles and separating the cells from the viral particles. The baculovirus viral particles are then recovered.
Claims11
A method of producing a recombinant membrane receptor protein, comprising:
- introducing a baculovirus expression vector encoding said membrane receptor protein into a host cell;
- culturing the resultant host cell for a sufficient time to permit expression of said membrane receptor protein and baculovirus viral particles;
- separating the cells from the viral particles;
- and
- recovering the baculovirus viral particles.
A method of producing recombinant membrane receptor from extracellular baculoviruses produced by a culture of cells infected with a recombinant baculovirus expressing a gene or cDNA encoding said membrane receptor, comprising:
- isolating said recombinant membrane receptor from extracellular baculoviruses produced by a culture of cells infected with a recombinant baculovirus expressing a gene or cDNA encoding said membrane receptor.
Dependencies are inferred from the claim text.