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US 7,078,178 B2Granted
Methods of altering calcium transport
AssigneeMatthias A. Hediger
InventorsMatthias A. Hediger, Edward M. Brown, Ji-Bin Peng
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Abstract
Methods of altering transmembrane calcium transport by altering expression of a calcium-transport protein are described.
Claims21
A method for modulating transmembrane calcium transport, the method comprising the steps of:
- a. providing a cell that expresses a nucleic acid encoding a protein that transports calcium across a membrane, the nucleic acid comprising the nucleotide sequence of SEQ ID NO;
- 1, or substitutions or modifications thereof, wherein the substituted or modified nucleic acid sequence encodes a protein that transports calcium across a cellular membrane wherein said protein is at least 75% identical to SEQ ID NO:2 but distinct from ECaC;
- and
- b. contacting the cell with a chemical that interacts with the protein so as to affect its calcium transmembrane transporting activity, thereby altering a rate of calcium transport across the membrane.
1 more element
A method of identifying the ability of a chemical to alter transmembrane calcium transport, the method comprising the steps of:
- a. providing a cell that expresses a nucleic acid encoding a protein that transports calcium across a membrane, the nucleic acid comprising the nucleotide sequence of SEQ ID NO:1, or substitutions or modifications thereof, wherein the substituted or modified nucleic acid sequence encodes a protein that transports calcium across a cellular membrane wherein said protein is at least 75% identical to SEQ ID NO:2 but distinct from ECaC, the cell expressing the protein in a membrane of the cell;
- b. contacting the membrane with at least one candidate antibody or nucleic acid;
- c. measuring the transmembrane calcium transport rate via the protein in the presence of the chemical;
- and
- d. comparing the measurement of step (c) with the transmembrane calcium transport rate via the protein in the absence of the chemical, wherein the comparison indicates whether the chemical has altered the transmembrane calcium transport.
2 more elements
Dependencies are inferred from the claim text.