Butyrylcholinesterase variants and methods of use
AssigneesBoard of Regents of the University of Nebraska, Applied Molecular Evolution, Inc.
InventorsOksana Lockridge, Jeffry D. Watkins
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Abstract
The invention provides four butyrylcholinesterase variants having increased cocaine hydrolysis activity as well as the corresponding encoding nucleic acids. The invention also provides libraries comprising butyrylcholinesterase variants having at least one amino acid alteration in one or more regions of butyrylcholinesterase and further having at least one butyrylcholinesterase variant exhibiting enhanced cocaine hydrolysis activity compared to butyrylcholinesterase. The invention further provides methods of hydrolyzing a cocaine-based butyrylcholinesterase substrate as well as methods of treating a cocaine-induced condition.
Claims2
A butyrylcholinesterase variant comprising an amino acid sequence comprising a sequence of amino acid residues 1 or 29 through 602, inclusive, of the amino acid sequence shown as SEQ ID NO:2, wherein said variant has tryptophan at amino acid position 356.
Dependencies are inferred from the claim text.