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US 7,049,127 B2Granted
Method of producing infectious reovirus
AssigneeOncolytics Biotech Inc.
InventorsBradley G. Thompson, Matthew C. Coffey
Life of this patent
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Abstract
A simple and efficient method of producing mammalian reovirus is developed using HEK 293 cells. The method provides for fast production of reovirus in high yield. Furthermore, this method provides for a simpler purification procedure of the produced reovirus.
Claims9
A method of producing reovirus, comprising the steps of:
- (a) contacting human embryo kidney 293 (HEK 293) cells with a reovirus under conditions which result in reoviral infection of said HEK 293 cells, wherein the reovirus is a reassorted reovirus;
- (b) incubating the culture of said infected cells for a period of time sufficient to allow for viral replication;
- and
- (c) harvesting the virus produced.
1 more element
Dependencies are inferred from the claim text.