US 6,969,603 B2 · Method for isolating DNA

Abstract

Method for isolating DNA contained in a biological sample. The method includes combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier, the solution having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution to form a bound DNA-carrier. The method also includes separating the DNA-bound carrier from other components. The method further includes dissociating the bound DNA from the DNA-binding carrier. The method still further includes recovering dissociated DNA.

Claims14

Dependent claims

A method for isolating DNA contained in a biological sample, comprising:

combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier which is not hydroxyapatite, the solution having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution of the DNA-containing biological sample, the salt, the cationic surfactant, and the DNA-binding carrier to form a bound DNA-carrier;
separating the DNA-bound carrier from other components;
dissociating the bound DNA from the DNA-binding carrier;
and
recovering dissociated DNA.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

09/984,742Filed 31 Oct 2001 · continuation

Priority

JP 9-25679522 Sep 1997

Published

US 2002/0025572 A128 Feb 2002

Granted

29 Nov 2005

Term ends

Not estimatedThe source records don’t support an estimate.

Classification3 codes

C12N 15/1017
Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses…
C12N 15/1006
Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses…
C12N
Microorganisms or enzymes

Family

PCT/JP98/04201Parent of this continuation · filed 18 Sep 1998

US 2002/0025572 A1Pre-grant publication · published 28 Feb 2002

Application09/508,119Parent of this continuation

US 6,342,387 B1Parent of this continuation

US 6,969,603 B2This patent

From the US filing history. Foreign family members aren’t listed.

Method for isolating DNA

Life of this patent

Method for isolating DNA

Life of this patent

Abstract

Claims14