Back to results
US 6,794,369 B2Granted
Methods, systems, and kits for intravascular nucleic acid delivery
AssigneePharmasonics, Inc.
InventorsChristopher M. H. Newman, Axel F. Brisken
Life of this patent
Estimating the term…
Abstract
Nucleic acid transfection of vascular smooth muscle cells is enhanced by the application of vibrational energy to the cells. By applying vibrational energy at frequency in the range from 1 kHz to 10 MHz and at an intensity in the range from 0.01 W/cm2 to 100 W/cm2, significant enhancement of the uptake of nucleic acids into vascular smooth muscle cells can be achieved.
Claims17
A method for intravascular nucleic acid delivery, said method comprising:
- providing a flexible catheter having a vibrational transducer disposed near its distal end;
- intravascularly positioning the distal end of the catheter at a target region within a blood vessel;
- delivering nucleic acids to vascular smooth muscle cells which line a wall of the blood vessel;
- and
- energizing the transducer to deliver vibrational energy to the wall at a frequency and intensity selected to enhance uptake of the nucleic acids by the smooth muscle cells, wherein expression of the nucleic acids in the cells exposed to the vibrational energy is at least two-fold greater than in comparable cells not exposed to vibrational energy.
2 more elements
A system comprising:
- a catheter having a vibratory interface surface;
- and
- a nucleic acid reagent which can be intravascularly delivered by the catheter.
Dependencies are inferred from the claim text.