Optimally labeled oligonucleotides
AssigneePoly Probe, Inc.
InventorThor W. Nilsen
Life of this patent
Estimating the term…
Abstract
A method for the preparation of optimally labeled oligonucleotides wherein label-conjugated nucleotide triphosphates are incorporated into a nucleic acid sequence in a defined repetitive manner which allows for the optimal specific detectability of the oligonucleotide. The oligonucleotides of the present invention are useful in the assay of a wide variety of nucleic acid sequences, specifically wherever labeled nucleic acid probes are desired.
Claims21
An oligonucleotide comprising a nucleotide sequence complementary to a primer, and a nucleotide repeat region downstream form said complementary sequence, wherein said nucleotide repeat region comprises at least one detectably labeled nucleotide, each of said at least one detectably labeled nucleotide containing the same detectable label, and wherein said nucleotide repeat region also comprises a plurality of nucleotides that are not detectably labeled.
An optionally labeled oligonucleotide, comprising a detectably labeled nucleic acid sequence and a nucleotide repeat region, wherein the nucleotide repeat region comprises at least one nucleotide that is conjugated to a detectable label, and a plurality of nucleotides that are not conjugated to a detectable label, wherein each of said at least one detectably labeled nucleotide contains the same detectable label.
Dependencies are inferred from the claim text.