Loading patent…
AssigneesThe Council of the Queensland Institute of Medical Research, Commonwealth Scientific and Industrial Research Organisation of Limestone Avenue, The University of Melbourne of Royal Parade, Walter and Elisa Hall Institute of Medical Research of Royal Melbourne Hospital, CSL Limited
InventorsScott Renton Burrows, Rajiv Khanna, Martina Alison Sherritt
Estimating the term…
The present invention provides cytotoxic Epstein-Barr virus (EBV) T-cell epitopes derived from EBV structural antigens. Preferred epitopes include YLLEMLWRL (SEQ ID NO:1), YFLEILWGL (SEQ ID NO:32), YLLEILWRL (SEQ ID NO:33), YLQQNWWTL (SEQ ID NO:6), LLLALLFWL (SEQ ID NO:2), LLVDLLWLL (SEQ ID NO:3), LLLIALWNL (SEQ ID NO:4), WLLLFLAIL (SEQ ID NO:5), TLLVDLLWL (SEQ ID NO:7), LLWLLLFLA (SEQ ID NO:8), ILLIIALYL (SEQ ID NO:9), VLFIFGCLL (SEQ ID NO:10), RLGATIWQL (SEQ ID NO:11), ILYFIAFAL (SEQ ID NO:15), SLVIVTTFV (SEQ ID NO:17), LMIIPLINV (SEQ ID NO:20), TLFIGSHVV (SEQ ID NO:24), LIPETVPYI (SEQ ID NO:26), VLQWASLAV (SEQ ID NO:27) and QLTPHTKAV (SEQ ID NO:29). The present invention also provides methods of treating or preventing EBV infection in subjects which involve administration of EBV cytotoxic T-cell epitopes.
An isolated peptide consisting of a cytotoxic T-cell epitope having an amino acid sequence selected from the group consisting of YLLEMLWRL (SEQ ID NO:1), YFLEILWGL (SEQ ID NO:32), YLLEILWRL (SEQ ID NO:33), YLQQNWWTL (SEQ ID NO:6), LLLALLFWL (SEQ ID NO:2), LLVDLLWLL (SEQ ID NO:3), LLLIALWNL (SEQ ID NO:4), WLLLFLAIL (SEQ ID NO:5), TLLVDLLWL (SEQ ID NO:7), LLWLLLFLA (SEQ NO:8), ILLIIALYL (SEQ ID NO:9), VLFIFGCLL (SEQ ID NO:10), RLGATIWQL (SEQ ID NO:11), ILYFIAFAL (SEQ ID NO:15), SLVIVTTFV (SEQ ID NO:17), LMIIPLINV (SEQ ED NO:20), TLFIGSHVV (SEQ ID NO:24), LIPETVPYI (SEQ ID NO:26), VLQWASLAV (SEQ ID. NO:27) and QLTPHTKAV (SEQ ID NO:29).
An isolated nucleic acid comprising a nucleotide sequence encoding a peptide consisting of a cytotoxic T-cell epitope having an amino acid sequence selected from the group consisting of YLLEMLWRL (SEQ ID NO:1), YFLEILWGL (SEQ ID NO:32), YLLEILWRL (SEQ ID NO:33), YLQQNWWTL (SEQ ID NO:6), LLLALLFWL (SEQ ID NO:2), LLVDLLWLL (SEQ ID NO:3), LLLIALWNL (SEQ ID NO:4), WLLLFLAIL (SEQ ID NO:5), TLLVDLLWL (SEQ ID NO:7), LLWLLLFLA (SEQ ID NO:8), ILLIIALYL (SEQ ID NO:9), VLFIFGCLL (SEQ ID NO:10), RLGATIWQL (SEQ ID NO:11), ILYFIAFAL (SEQ ID NO:15), SLVIVTTFV (SEQ ID NO:17), LMIIPLINV (SEQ ID NO:20), TLFIGSHVV (SEQ ID NO:24), LIPETVPYI (SEQ ID NO:26), VLQWASLAV (SEQ ID NO:27) and QLTPHTKAV (SEQ ID NO:29).
An isolated polypeptide comprising a plurality of isolated Epstein-Barr virus (EBV) CTL epitopes selected from the group consisting of YLLEMLWRL (SEQ ID NO:1), YFLEILWGL (SEQ ID NO:32), YLLEILWRL (SEQ ID NO:33), YLOONWWTL (SEQ ID NO:6). LLLALLFWL (SEQ ID NO:2), LLVDLLWLL (SEQ ID NO:3), LLLIALWNL (SEQ ID NO.4), WLLLFLAIL (SEQ ID NO:5). TLLVDLLWL (SEQ ID NO:7), LLWLLLFLA (SEQ ID NO:8). ILLIIALYL (SEQ ID NO:9). VLFIFGCLL (SEQ ID NO:10). RLGATIWQL (SEQ ID NO:11), ILYFIAFAL (SEQ ID NO:15), SLVIVTTFV (SEQ ID NO:17), LMIIPLINV.(SEQ ID NO:20). TLFIGSHVV (SEQ ID NO:24). LIPETVPYI (SEQ ID NO:26). VLQWASLAV (SEQ ID NO:27) and QLTPHTKAV (SEQ ID NO:29).
A method of preparing a composition for use in inducing CTLs in a subject, the method comprising admixing at least one peptide selected from the group consisting of YLLEMLWRL (SEQ ID NO:1), YFLEILWGL (SEQ ID NO:32), YLLEILWRL (SEQ ID NO:33), YLOQNWWTL (SEQ ID NO:6), LLLALLFWL (SEQ ID NO:2), LLVDLLWLL (SEQ ID NO:3), LLLIALWNL (SEQ ID N0:4), WLLLFLAIL (SEQ ID NO:5), TLLVDLLWL (SEQ ID NO;7), LLWLLLFLA (SEQ ID NO:8), ILLIIALYL (SEQ ID NO:9). VLFIFGCLL (SEQ ID NO:10), RLGATIWQL (SEQ ID NO:11), ILYFIAFAL (SEQ ID NO:15). SLVIVTTFV (SEQ ID NO:17). LMIIPLINV (SEQ ID NO:20). TLFIGSHVV (SEQ ID NO:24). LIPETVPYI (SEQ ID NO:26), VLQWASLAV (SEQ ID NO:27) and OLTPHTKAV (SEQ ID NO:29). with a pharmaceutically acceptable carrier, diluent or excipient.
Dependencies are inferred from the claim text.