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US 6,720,417 B1Granted
Method and device for refining nucleic acids
AssigneeRoche Diagnostics GmbH
InventorThomas Walter
Life of this patent
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Abstract
A device for isolating nucleic acids which reduces the transfer of contaminants during nucleic acid isolation methods which is composed of two vessels which are linked by a closing element in which a material that binds nucleic acids is placed. The nucleic acid can for example be bound to the material by tipping the device.
Claims11
A method for isolating nucleic acids from a sample, comprising:
- a) taking the sample into a first vessel through an opening in the first vessel,
- b) closing the opening in the first vessel with a closing element, wherein the closing element comprises a glass fleece or a membrane comprising metal oxides, metal mixed oxides, silica gel, aluminum oxide, zeolite, titanium oxide or zirconium oxide, in the form of fibres or particles, on a side facing the opening, means for attaching the element on the first vessel and, on an other side, means for attaching the element to a second vessel, and
- c) transferring the sample through the material into a second vessel wherein the second vessel is attached on the other side of the closing element, and wherein the sample is transferred by applying an underpressure in the second vessel;
- wherein said glass fleece or membrane is not functionalized with ion-exchange substituents.
1 more element
Dependencies are inferred from the claim text.