US 6,720,193 B2 · Analysis of free analyte fractions by rapid affinity chromatography

Abstract

The invention is generally directed toward an analytical method to determine the concentration of the free analyte fraction in a sample. More particularly, the method encompasses applying a sample comprising a free and bound analyte fraction to an affinity column capable of selectively extracting the free fraction in the millisecond time domain. The signal generated by the free fraction is then quantified by standard analytical detection techniques. The concentration of the free fraction may then be determined by comparison of its signal with that of a calibration curve depicting the signal of known concentration of the same analyte.

Claims24

Dependent claims

A method to determine the concentration of a free analyte fraction in a sample, the sample comprising a biological fluid having a bound analyte fraction and the free analyte fraction, the method comprising:

(a) applying, the sample to an affinity column having an active layer which selectively binds free analyte relative to bound analyte wherein the active layer separates the free analyte fraction from the bound analyte fraction of the sample in the millisecond time domain;
(b) detection of signal from the free analyte fraction separated from the sample;
and
(c) determining the concentration of free analyte present in the sample.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

09/794,857Filed 27 Feb 2001 · continuation in part

Priority

5 Feb 2001

Published

US 2002/0146840 A117 Oct 2002

Granted

13 Apr 2004

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

G01N 33/54393
With an insoluble carrier for immobilising immunochemicals: improving reaction conditions or stability, e.g. by coating or irradiation of surface,…
G01N 33/54313
With an insoluble carrier for immobilising immunochemicals: the carrier being characterised by its particulate form
G01N 33/54366
With an insoluble carrier for immobilising immunochemicals: apparatus specially adapted for solid-phase testing
G01N 2030/527
Structural properties: sorbent material in form of a membrane

Family

Application09/776,800Parent of this continuation-in-part · filed 5 Feb 2001

US 2002/0151086 A1Pre-grant publication · published 17 Oct 2002

US 2002/0146840 A1Published application

US 6,500,671 B2Related patent

US 6,720,193 B2This patent

From the US filing history. Foreign family members aren’t listed.

Analysis of free analyte fractions by rapid affinity chromatography

Life of this patent

Analysis of free analyte fractions by rapid affinity chromatography

Life of this patent

Abstract

Claims24