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US 6,720,161 B1Granted
Immobilized substrate, separation gels and method for detecting enzymatic activity
AssigneeGesellschaft fuer Biotechnologische Forschung mbH
InventorsRonald Frank, Werner Tegge
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Abstract
The present invention refers to a method for attaching substrate molecules to gel matrices, characterized in that a substrate is covalently bound to a monomeric or oligomeric constitutional unit of a gel and the resultant product is polymerized. The present invention further refers to gels obtainable by this process.
Claims14
A method for separating and detecting protein kinase activities by conducting the following steps in the given order:
- (a) covalently binding a substrate to a monomeric or oligomeric constitutional unit of a gel;
- (b) polymerizing the resultant product to form a gel matrix of a separating gel;
- (c) electrophoretically separating the protein kinase activities to be separated using the separating gel, and optionally renaturating;
- and
- (d) detecting, either by labelling with a detectable label or destroying a label present, by reacting the substrate which is covalently bound in the gel with the separate protein kinase activity/activities.
2 more elements
A method of separating and detecting protein kinase activities by conducting the following steps in the given order:
- (a) using a separating gel which is obtained by covalently binding a substrate to a monomeric or oligomeric constitutional unit of a gel and polymerizing the resultant product to form a gel matrix of a separating gel;
- (b) electrophoretically separating the protein kinase activities to be separated using the separating gel, optionally renaturating;
- and
- (c) detecting, either by labelling with a detectable label or destroying a label present, by reacting the substrate which is covalently bound in the gel with the separate protein kinase activity/activities.
1 more element
Dependencies are inferred from the claim text.