Anti-hirudin polyclonal antibodies and their use for the identification, immunopurification and quantitative determination of hirudin
AssigneeFarmitalia Carlo Erba S.p.A.
InventorsAntonio Molinari, Marco Gerna, Carla Giorgetti, Jacqueline Lansen, Romeo Roncucci
Life of this patent
Estimating the term…
Abstract
A method of making antibodies to hirudin by immunizing with an immunogenic composition containing polymerized hirudin monomers in the absence of carrier protein is taught.
Claims5
An immunogen consisting essentially of a set of hirudin polymers being devoid of any heterologous carrier protein and having sufficient immunogenicity, in the absence of conjugation to said carrier protein, to elicit an antibody which binds specifically to a natural or recombinant hirudin, and, the majority of said polymers having at least three monomer units, said monomers selected from the group consisting of HV1 (SEQ ID NO:1), HV2 (SEQ ID NO:2), and HV3 (SEQ ID NO:3).
Dependencies are inferred from the claim text.