Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system for performing biological fluid assays

A microsystem platform for performing an assay to detect an analyte in a fluid sample, comprising:

• a) a rotatable platform, comprising a substrate having a first flat, planar surface and a second flat, planar surface opposite thereto, each surface comprising a center about which the platform is rotated, wherein the first surface comprises in combination
• b) an entry port comprising a depression in the first surface having a volumetric capacity of about 1 to about 150 μL, that is fluidly connected with
• c) a capillary microchannel having a cross-sectional diameter of less than 800 microns, that is further fluidly connected with
• d) an assay chamber fluidly connected with the entry port, the assay chamber further comprising
• i) a porous matrix comprising reagents for performing an analyte detection assay, wherein the porous matrix has an average pore size that prevents cells from entering the matrix;
• and
• wherein a fluid sample applied to the entry port is delivered to the assay chamber through the capillary microchannel by rotation of the platform, and wherein delivery of the fluid sample to the assay chamber initiates the analyte detection assay.