Nucleic acid coating compositions and methods
AssigneeBioSurface Engineering Technologies, Inc.
InventorsPaul O. Zamora, Ray Tsang, Shigemasa Osaki
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Abstract
A nucleic acid coating composition including a polyanion bound, directly or through one or more intermediates, to a medical device surface, with a condensate comprising a polycation and nucleic acid bound to the polyanion, devices incorporating such coating compositions, and methods for making. In one embodiment, a silyl-heparin complex is provided, bound to a medical device surface by hydrophobic interaction with the silyl moiety, with a polycation and nucleic acid condensate bound to the heparin by electrostatic interaction.
Claims33
A nucleic acid-containing coating composition for contacting surfaces of medical devices, said composition comprising a polyanion substrate having bound thereto by non-covalent means a condensate comprising a polycation and nucleic acid.
A coating composition for contacting surfaces of medical device, said composition comprising a molecule of Formula I:
- wherein
- R1 is an C1-18 alkyl or C6-32 aryl group,
- each R2 is independently selected from the group consisting of C1-18 alkyl and C6-32 aryl
- R3 is N or O,
- n is a number from 1 to 10, and
- x is a number from 1 to about 30,
- bound to a heparin-activity molecule via a covalent bond, thereby forming a silyl-heparin covalent complex, with a condensate comprising a polycation and nucleic acid directly and non-covalently bound to the heparin-activity molecule.
A medical device with at least one contacting surface for contacting bodily fluids, the surface having coated thereon a coating composition comprising a molecule of Formula I:
- wherein
- R1 is an C1-18 alkyl or C6-32 aryl group,
- each R2 is independently selected from the group consisting of C1-18 alkyl and C6-32 aryl,
- R3 is N or O,
- n is a number from 1 to 10, and
- x is a number from 1 to about 30,
- directly bound to a heparin-activity molecule via covalent bonding, thereby forming a silyl-heparin covalent complex, with a condensate comprising a polycation and nucleic acid directly and non-covalently bound to the heparin-activity molecule.
A method for coating a contacting surface of a medical device with a nucleic acid-containing coating composition, comprising:
- providing a hydrophobic silyl moiety of Formula I
- wherein
- R1 is an C1-18 alkyl or C6-32 aryl group,
- each R2 is independently selected from the group consisting of C1-18 alkyl and C6-32 aryl,
- R3 is N or O, and
- n is a number from 1 to 10
- binding said silyl moiety to a heparin-activity molecule via covalent bonding, wherein x is from 1 to about 30 for each heparin-activity molecule, thereby forming a silyl-heparin complex,
- attaching the silyl-heparin complex to the contacting surface by hydrophobic interaction, and
- attaching a condensate comprising a polycation and a nucleic acid to the heparin-activity molecule.
Dependencies are inferred from the claim text.