Back to results
US 6,555,391 B1Granted
Methods of conditioning an affinity resin after storage
AssigneeBaxter International Inc.
InventorsSusan L. Bernhard, Robert Toso, Van Taiariol
Life of this patent
Estimating the term…
Abstract
This invention relates methods for conditioning affinity chromatography resins to decrease leaching of the ligand during purification. The methods involve incubating the resin in a buffered solution of a hydroxyalkylamine compound (e.g., ethanolamine) prior to use of the resin for an affinity purification. The treatment removes unstably bound ligand from the resin.
Claims20
A method for decreasing leaching of a ligand from an affinity resin, the method comprising:
- (a) contacting an affinity resin with an effective amount of a hydroxyalkyl amine compound that comprises at least one hydroxyl moiety, wherein said affinity resin comprises a ligand conjugated to a chromatographic support and has been stored for at least 48 hours after coupling said ligand to said chromatographic support;
- and
- (b) incubating the affinity resin in the hydroxyalkyl amine compound for a time sufficient to remove unstably attached ligand from said affinity resin;
- wherein when said affinity resin is used to purify a molecule to which the ligand specifically binds, less ligand leaches from said affinity resin than when an aliquot of the affinity resin that has not been treated as described in (a) is used for the purification of the molecule.
1 more element
Dependencies are inferred from the claim text.