Sheets of cultured epithelial cells used for wound repair and their cryopreservation
AssigneeCeladon Science, LLC
InventorsWalid Kuri-Harcuch, Federico Castro Munozledo, Luis Salazar-Olivio, Meytha Marsch-Moreno
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Abstract
Methods of cryopreserving sheets of cultured epithelial cells are disclosed. The sheets of cultured epithelial cells are used for wound repair. The invention permits the use of preserved tissue without the need to wash away cryopreservation components. The sheets are frozen in contact with a cryoprotectant solution containing a cryoprotectant such as a monosaccharide or a disaccharide.
Claims13
A frozen sheet of cultured mammalian epithelial cells which after being thawed does not need to be washed prior to applying the sheet to a wound, comprising:
- a sheet of cultured mammalian epithelial cells frozen in contact with a cryoprotectant solution consisting essentially of a cryoprotectant selected from the group consisting of monosaccharides, disaccharides, and mixtures thereof, wherein the cryoprotectant solution is free of materials that would interfere with wound healing and free of materials that must be washed from the sheet prior to application of the sheet to a wound bed.
A method for preserving a sheet of cultured mammalian epithelial cells for later application to a wound bed, comprising the following steps:
- (a) incubating said sheet of cultured mammalian epithelial cells in a cryoprotectant solution consisting essentially of a cryoprotectant selected from the group consisting of monosaccharides, disaccharides, and mixtures thereof, wherein said cryoprotectant solution is free of materials that would interfere with wound healing and free of materials that must be washed from the cells prior to application of the sheet to a wound bed, and
- (b) freezing said sheet of cultured mammalian epithelial cells while in said cryoprotectant solution in contact with said cryoprotectant, whereby upon thawing the sheet does not need to be washed prior to applying the sheet to a wound bed.
Dependencies are inferred from the claim text.