Purification of von-Willebrand factor by cation exchanger chromatography
AssigneeBaxter Aktiengesellschaft
InventorsBernhard Fischer, Öyvind L. Schönberger, Artur Mitterer, Christian Fiedler, Friedrich Dorner, Johann Eibl
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Abstract
Disclosed are a method of recovering vWF in which vWF at a low salt concentration is bound to a cation exchanger and vWF having a high specific activity is recovered by fractionated elution, as well as a preparation having purified vWF obtainable by this method.
Claims22
Delete “vWF” and Insert “von Willebrand factor (vWF)” in line 1 of the claim.
A preparation comprising purified von Willebrand factor (vWF) containing high-molecular weight vWF multimers, obtainable from a vWF-containing protein solution by cation exchange chromatography.
A method of treating a patient suffering from a disorder, selected from the group consisting of phenotypic hemophilia and von Willebrand Syndrome, by administering an effective dose of a von Willebrand factor (vWF)-containing preparation to said patient, said vWF-containing preparation comprising purified vWF containing high-molecular weight vWF multimers with a factor VHII content of 1% (total protein) or less, obtained from a vWF-containing protein solution by cation exchange chromatography.
Dependencies are inferred from the claim text.