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US 6,409,774 B1Granted
Electrophoresis-assisted staining of materials
AssigneeResolution Sciences Corporation
InventorsRussell L. Kerschmann, Ronald Odom, Thomas Kuwahara, Mark Reddington
Life of this patent
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Abstract
A method for en bloc staining a biological sample by (a) immersing the sample in a staining solution including an ionically conductive solution and a charged stain molecule or stain precursor that associates with a component of the sample; and (b) applying an electric field across the staining solution, whereby the stain molecule penetrates into the sample. Staining times are reduced.
Claims20
A method for en bloc staining a sample, said method comprising the steps of:
- a) immersing the sample in a staining solution including (i) an ionically conductive solution, and (ii) a charged stain molecule and/or stain precursor that associates with a component of the sample;
- b) applying an electric field across the staining solution, whereby the stain molecule penetrates into the sample to produce an en bloc stained sample;
- and
- c) embedding said en bloc stained sample.
1 more element
A method for reducing unbound stain molecule in a sectioned tissue, said method comprising the steps of:
- a) immersing the a tissue sample in a staining solution including an ionically conductive solution and a stain precursor that associates with a component of the sample;
- and
- b) applying an electric field across the staining solution, whereby penetration of the stain precursor into the sample and emigration of unbound stain precursor from the sample occurs.
Dependencies are inferred from the claim text.