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AssigneeHer Majesty the Queen in right of Canada, as represented by the Minister of Natural Resources Canadian Forest Service
InventorsAbul K. M. Ekramoddoullah, Douglas W. Taylor, Xueshu Yu, Santosh Misra
Estimating the term…
A gene coding for Pin m III, which is associated with overwintering and frost hardiness in western white pine, has been cloned and characterized. The gene contains an open reading frame of 486 base pairs, encoding a protein of 161 amino acid residues, with a calculated molecular weight of 18002.30 daltons. The predicted isoelectricl point is 5.534. The gene is transcriptionally regulated with the highest expression in fall and winter months. The protein Pin m III is high in infected tissue such as canker. Accordingly, the gene can be used to determine frost hardiness and blister resist fungus susceptibility in conifers.
A nucleic acid sequence encoding the protein Pin m III having the amino acid sequence of SEQ ID NO:
The isolated protein Pin m III comprising the open reading frame, from bases 55 to 537 (amino acids 1-161) of SEQ ID NO:
A method of determining the frost hardiness of a conifer seedling comprising detecting the amount of a protein having the open reading frame, from bases 55 to 537 (amino acids 1-161) of SEQ ID NO:
A method of detecting the presence of blister rust fungus in a conifer comprising measuring the amount of a protein having the open reading frame, from bases 55 to 537 (amino acids 1-161) of SEQ ID NO:
Dependencies are inferred from the claim text.