Gene encoding oxalate decarboxylase from aspergillus phoenices
AssigneePioneer Hi-Bred International, Inc.
InventorsChristopher J. Scelonge, Dennis L. Bidney
Life of this patent
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Abstract
A novel nucleic acid sequence encoding Aspergillus phoenices oxalate decarboxylase (APOXD) has been determined, as well as the encoded amino acid sequence. The gene and its encoded protein are useful in degrading oxalate, in diagnostic assays of oxalate, and as a selectable marker.
Claims29
An isolated nucleic acid encoding an oxalate decarboxylase enzyme from Aspergillus phoenices, said nucleic acid selected from the group consisting of:
- a) nucleotide 1 to 1437 of the nucleic acid shown in SEQ ID NO:
- 1;
- b) nucleotide 171 to 1437 of the nucleic acid shown in SEQ ID NO:
- 1;
- c) a nucleic acid having the sequence of the Aspergillus phoenices insert in the plasmid ATCC No. 97959;
- and
- d) a nucleic acid encoding an oxalate decarboxylase having the amino acid sequence set forth in SEQ ID NO:
- 2 or SEQ ID NO:
- 5.
An isolated nucleic acid encoding a signal peptide comprising nucleotide 24 to 101 of SEQ ID NO:
- 1.
An isolated nucleic acid comprising the nucleic acid sequence shown in SEQ ID NO:
- 1.
A vector for delivery of a nucleic acid to a host cell, the vector comprising the nucleic acid shown in SEQ ID NO:
- 1.
A microbial or plant host cell transformed with the nucleic acid shown in SEQ ID NO:
- 1.
A plant cell transformed with a nucleic acid comprising the nucleic acid shown in SEQ ID NO:
- 1.
A plant having stably incorporated within its genome a nucleic acid comprising the nucleic acid shown in SEQ ID NO:
- 1.
A method for degrading oxalic acid comprising transforming a plant or plant cell with the nucleic acid shown in SEQ ID NO:
- 1 and inducing expression of said nucleic acid for a time sufficient to degrade oxalic acid.
Dependencies are inferred from the claim text.