Pesticidal toxins and genes from Bacillus laterosporus strains
AssigneeMycogen Corporation
InventorsH. Ernest Schnepf, Kenneth E. Narva, Brian A. Stockhoff, Stacey Finstad Lee, Mikki Walz, Blake Sturgis
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Abstract
Disclosed and claimed are novel toxins and genes obtainable from Bacillus laterosporus isolates disclosed herein. In preferred embodiments, the subject genes and toxins are used to control Western corn rootworm.
Claims22
An isolated polynucleotide encoding a toxin that is active against a corn rootworm pest wherein said toxin is from E. coli clone MR957 having accession number NRRL B-30048, said clone comprising a DNA segment encoding said toxin, wherein approximately 1 kilobase of said DNA segment is amplifiable by polymerase chain reaction using the primers of SEQ ID NO:3 and SEQ ID NO:4.
An isolated polynucleotide that encodes a toxin that is active against a corn rootworm pest, wherein said toxin comprises the amino acid sequence of SEQ ID NO:7 or a segment thereof that retains the toxin activity of the toxin defined by SEQ ID NO:7.
A plant or bacterial cell comprising an isolated polynucleotide that encodes a toxin that is active against a corn rootworm pest, wherein said toxin is from E. coli clone MR957 having accession number NRRL B-30048, said clone comprising a DNA segment encoding said toxin, wherein an approximately 1 kilobase portion of said DNA segment is amplifiable by polymerase chain reaction using the primers of SEQ ID NO:3 and SEQ ID NO:4.
An isolated polynucleotide that encodes a toxin that is active against a corn rootworm pest, wherein said toxin comprises an amino acid sequence that has at least 90% identity with SEQ ID NO:7 and retains the toxin activity of the toxin defined by SEQ ID NO:7.
A plant or bacterial cell comprising an isolated polynucleotide that encodes a toxin that is active against a corn rootworm pest, wherein said toxin comprises an amino acid sequence of SEQ ID NO:7 or a segment thereof that retains the toxin activity of the toxin defined by SEQ ID NO:7.
A plant or bacterial cell comprising an isolated polynucleotide that encodes a toxin that is active against a corn rootworm pest, wherein said toxin comprises an amino acid sequence that has at least 90% identity with SEQ ID NO:7 and retains the toxin activity of the toxin defined by SEQ ID NO:7.
Dependencies are inferred from the claim text.