Adenovirus-medicated gene transfer to cardiac and vascular smooth muscle
AssigneeArch Development Corporation
InventorsJeffrey M. Leiden, Eliav Barr
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Abstract
The present invention relates to the use of adenovirus-mediated gene transfer to regulate function in cardiac and vascular smooth muscle cells. A recombinant adenovirus comprising a DNA sequence that codes for a gene product is delivered to a cardiac or vascular smooth muscle cell and the cell is maintained until that gene product is expressed. Delivery is direct injection into a muscle cell or infusing a pharmaceutical composition containing an adenovirus virus vector construct intravascularly.
Claims42
A process for delivering a nucleic acid encoding a gene product to a vascular smooth muscle cell comprising the steps of:
- a) infusing an adenovirus vector construct comprising a coding sequence that encodes said gene product into the blood flow of an artery perfusing said vascular smooth muscle cell wherein said vector drives expression of said gene product in said muscle cell;
- and
- b) obtaining expression of said gene product in said vascular smooth muscle cell.
A process for delivering a nucleic acid encoding a gene product to a cardiac muscle cell comprising the steps of:
- a) infusing an adenovirus vector contruct comprising a coding sequence that encodes said gene product into the blood flow of a coronary artery or coronary sinus perfusing said cardiac muscle cell, said vector driving expression of said gene product in said muscle cell;
- and
- b) obtaining expression of said gene product in said cardiac muscle cell.
A process of transforming a vascular smooth muscle cell with a polynucleotide that encodes a particular gene sequence comprising infusing an adenovirus vector construct containing the polynucleotide into the blood flow of an artery perfusing said vascular smooth muscle cell.
A process of transforming a cardiac muscle cell with a polynucleotide that encodes a particular gene sequence comprising infusing an adenovirus vector construct containing the polynucleotide into the blood flow of a coronary artery or coronary sinus perfusing said cardiac muscle cell.
Dependencies are inferred from the claim text.