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OwnerChristopher C. Barnett, Colin Mitchinson +2inventors
InventorsChristopher C. Barnett, Colin Mitchinson, Scott D. Power, Carol A. Requadt
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Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.
A mutant alpha amylase derived from Bacillus, the mutant alpha amylase having a substitution of an amino acid selected from the group consisting of threonine, leucine, alanine, arginine, asparagine, aspartic acid, glutamic acid, glycine, isoleucine, lysine, phenylalanine, proline, serine, valine, histidine and glutamine or a deletion of a methionine residue at a position equivalent to M197 Bacillus licheniformis alpha amylase.
A mutant alpha amylase derived from Bacillus, the mutant alpha amylase having a substitution of an amino acid selected from the group consisting of leucine, threonine, asparagine, aspartate, serine, valine, and isoleucine for a methionine residue at a position equivalent to M15 in Bacillus licheniformis alpha amylase.
An alpha-amylase comprising an amino acid sequence corresponding to SEQ ID No. 37.
A mutant alpha amylase derived from Bacillus, the mutant alpha amylase having at least two substitutions at positions equivalent to M15, W138 and/or M197 in Bacillus licheniformis alpha amylase, wherein said substitutions comprise M15T, W138Y and/or M197T.
A mutant alpha-amylase having enhanced oxidative stability, the mutant alpha-amylase being derived from Bacillus and comprising a substitution of methionine with alanine, arginine, glycine, lysine, phenylalanine, proline, threonine or valine at an amino acid residue equivalent to M197 in Bacillus licheniformis alpha-amylase.
Dependencies are inferred from the claim text.