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US 6,297,012 B1Granted
Cytoplasmic male sterility DNA factor and utilization thereof
AssigneeSumitomo Chemical Company, Limited
InventorsYuki Nakajima, Toshiya Yamamoto, Kenji Oeda
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Abstract
There is provided a cytoplasmic male sterility DNA factor, a method of discrimination which enables confirmation of the cytoplasmic male sterility in a short time.
Claims3
A method of identifying a plant containing a cytoplasmic male sterility DNA factor, said DNA comprising a nucleotide sequence from the group consisting of:
- (a) a nucleotide sequence of 171 bp characterized by a restriction map shown by FIG. 1 and having restriction sites, HinfI (5 bp), MboI (24 bp), MboI (43 bp) and HapII (122 bp);
- (b) a nucleotide sequence shown by SEQ ID NO:
- 1;
- (c) a nucleotide sequence encoding the amino acid sequence depicted in SEQ ID NO:
- 2;
- and
- (d) a nucleotide sequence hybridizing with a nucleotide sequence of (b) or (c), which method comprises:
- carrying out a PCR-amplification of a genomic DNA of a plant using, as a primer, an oligonucleotide which can amplify a cytoplasmic male sterility DNA factor as defined above or a part of it or a DNA containing said DNA or an equivalent thereof,
- separating the amplified genomic DNA by electrophoresis, and then
- visually detecting the amplified genomic DNA, wherein the detection of said amplified genomic DNA is indicative of a plant containing said cytoplasmic male sterility DNA factor.
7 more elements
A method of identifying a plant containing a cytoplasmic male sterility DNA factor, said DNA comprising a nucleotide sequence from the group consisting of:
- (a) a nucleotide sequence of 171 bp characterized by a restriction map shown by FIG. 1 and having restriction sites, HinfI (5 bp), MboI (24 bp), MboI (43 bp) and HapII (122 bp);
- (b) a nucleotide sequence shown by SEQ ID NO:
- 1;
- (c) a nucleotide sequence encoding the amino acid sequence depicted in SEQ ID NO:
- 2;
- and
- (d) a nucleotide sequence hybridizing with a nucleotide sequence of (b) or (c), which method comprises:
- conducting Southern or Northern hybridization on a genomic DNA or RNA of a plant as a sample for analysis using, as a probe, a cytoplasmic male sterility DNA factor as defined above or an equivalent thereof, wherein the hybridization of said genomic DNA or RNA and said probe is indicative of a plant containing said cytoplasmic male sterility DNA factor.
5 more elements
Dependencies are inferred from the claim text.