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AssigneeThe Regents of the University of California
InventorsDavid A. Pot, Lewis T. Williams, Anne Bennett Jefferson, Philip W. Majerus
Estimating the term…
The present invention generally relates to novel GRB2 associating proteins and nucleic acids which encode these protein. In particular, these novel proteins possess inositol polyphosphate 5-phosphatase and phosphatidylinositol 5-phosphatase activities, important in growth factor mediated signal transduction. As such, the proteins, nucleic acids encoding the proteins, cells capable of expressing these nucleic acids and antibodies specific for these proteins will find a variety of uses in a variety of screening, therapeutic and other applications.
An isolated polypeptide comprising a catalytically active fragment of an amino acid sequence of FIG. 10 (SEQ ID NO:2), wherein said fragment comprises an inositol polyphosphate 5-phosphatase activity such that said fragment hydrolyzes a 5-phosphate from Ins(1,3,4,5)P4 and PtdIns(3,4,5)P3, but not Ins(1,4,5)P3 or Ptdlns(4,5)P2.
A kit for determining whether a test compound is an agonist or antagonist of GA5Ptase activity, comprising:
An isolated polypeptide, said polypeptide being immunologically cross-reactive with an antibody to a fragment of a GA5Ptase protein which comprises an amino acid sequence shown in FIG. 10 (SEQ ID NO:2), wherein said fragment has an inositol polyphosphate 5-phosphatase activity such that said fragment hydrolyzes a 5-phosphate from Ins(1,3,4,5)P4 and PtdIns(3,4,5)P3, but not Ins(1,4,5)P3 or PtdIns(4,5)P2.
An isolated polypeptide comprising a GRB2-associating fragment of amino acid sequence of FIG. 10 (SEQ ID NO:2).
Dependencies are inferred from the claim text.