Separating materials for chromatography and electrophoresis applications comprising regiodefined functionalised cyclodextrins chemically bonded to a support via urethane functionalities

A method for chromatographic or electrophoretic separation of a mixture comprising, respectively, contacting a sample comprising said mixture with or applying a sample comprising said mixture to a separation support material, said separation support material being one produced by a process comprising any one of the following steps:

• (a) reacting a regiodefined mono-azido perfunctionalized cyclodextrin or a regiodefined di-azido perfunctionalized cyclodextrin with a support that carries free NH2 groups;
• (b) reacting a regiodefined mono-azido perfunctionalized cyclodextrin or a regiodefined di-azido perfunctionalized cyclodextrinwith an alkenyl amine to produce a product comprising a C═C group linked to a side chain via a urethane linkage, further hydrosilating the C═C group of said product with an active silane, and chemically immobilizing the product onto the surface of a support;
• (c) reacting a regiodefined mono-azido perfunctionalized cyclodextrin or a regiodefined di-azido perfunctionalized cyclodextrin with an aminosilane containing at least one further reactive group to form a product, and chemically immobilizing the product onto a surface of a support;
• and
• (d) reducing a regiodefined mono-azido perfunctionalized cyclodextrin or a regiodefined di-azido perfunctionalized cyclodextrin to a monoamino or diamino perfunctionalized cyclodextrin, and then anchoring the mono- or diamino perfunctionalized cyclodextrin onto the surface of a support by a coupling reagent, and, in the case of electrophoretic separation, applying an electric field.