Analysis of carbohydrates
AssigneeAmersham International plc
InventorsPeter Jackson, William Jonathan Cummins, Richard West, John Anthony Smith, Mark Samuel Jonathan Briggs
Life of this patent
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Abstract
The present invention discloses a method of separating or distinguishing carbohydrate substances. More particularly the method includes using fluorescent labeling reagents which have a positive charge when bound to a carbohydrate, involves separating the labeled carbohydrate substances, such as by performing electrophoresis to cause differential migration of different labeled carbohydrate substances, or by isoelectric focusing in a pH gradient. The fluorescent labeling reagents are preferably cyanine dyes.
Claims13
A method of separating carbohydrate substances, comprising contacting the carbohydrate substances with two or more different fluorescent labeling reagents to bind said labeling reagents to said carbohydrate substances, wherein each of said two or more reagents comprise a reactive group capable of binding to a carbohydrate, and wherein each reagent carries a positive charge which is different from that carried by any other of the different reagents, and separating the carbohydrate substances by virtue of the different positive charges on the labeling reagents.
A method of separating carbohydrate substances that have been cleaved or released by hydrazine, comprising the step of labeling the carbohydrate substances by binding two or more different fluorescent labeling reagents to said carbohydrate substances, wherein each said labeling reagent has a reactive group capable of binding to a carbohydrate, and wherein each reagent carries a positive charge which is different from that carried by any other of the different reagents, and separating the carbohydrate substances by virtue of the different positive charges on the labeling reagents.
A method of separating carbohydrate substances comprising labeling the carbohydrate substances by binding two or more different fluorescent labeling reagents to aliquots of said carbohydrate substances, wherein each of said reagents has a reactive group capable of binding to a carbohydrate, and wherein each reagent carries a positive charge which is different from that carried by any other of the different reagents, and separating the carbohydrate substances by virtue of the different positive charges on the labeling reagents.
Dependencies are inferred from the claim text.