Compounds
AssigneeSmithKline Beecham Corporation
InventorsMichael Terence Black, John Edward Hodgson, David Justin Charles Knowles, Richard O Nicholas, Robert King Stodola
Life of this patent
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Abstract
The invention provides RF-1 polypeptides and DNA (RNA) encoding RF-1 polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing RF-1 polypeptides to screen for antibacterial compounds.
Claims21
An isolated polynucleotide segment comprising a polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence, wherein the polynucleotide sequence is at least 95% identical to SEQ ID NO:1, wherein the polynucleotide sequence is not genomic DNA and wherein the polynucleotide sequence detects Streptococcus pneumoniae by hybridization.
An isolated polynucleotide segment comprising a polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence, wherein the polynucleotide sequence comprises SEQ ID NO:1, wherein the polynucleotide sequence is not genomic DNA and wherein the polynucleotide sequence detects Streptococcus pneumoniae by hybridization.
An isolated polynucleotide segment comprising a polynucleotide sequence, wherein the polynucleotide sequence hybridizes to the full complement of SEQ ID NO:1, wherein the hybridization conditions include incubation at 42° C. in a solution comprising:
- 50% formamide, 5× SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5× Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing in 0.1 × SSC at 65° C.;
- and, wherein the polynucleotide sequence is at least 95% identical to SEQ ID NO:1, wherein the polynucleotide sequence is not genomic DNA and wherein the polynucleotide sequence detects Streptococcus pneumoniae by hybridization.
An isolated polynucleotide segment comprising a polynucleotide sequence, wherein the polynucleotide sequence encodes a polypeptide comprising SEQ ID NO:2, and wherein the polynucleotide sequence is not genomic DNA.
An isolated polynucleotide segment comprising a polynucleotide sequence, wherein the polynucleotide sequence encodes a polypeptide consisting of SEQ ID NO:2, and wherein the polynucleotide sequence is not genomic DNA.
Dependencies are inferred from the claim text.