CD123+ dendritic cells in blood and lymphoid tissues
OwnerJohanna Olweus, Fridtjof Lund-Johanseninventors
Life of this patent
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Abstract
Dendritic cells (DCs) are the primary antigen presenting cells during the initiation of T cell-dependent immune responses. The cells originate from the bone marrow and have been suggested to represent a distinct cell lineage. However, distinct DC precursors have not been identified in bone marrow, and mature monocytes can also give rise to DCs. The instant invention presents a distinct DC precursor among bone marrow CD34+ cells. The cells express high levels of the interleukin-3 receptor α chain and CD4 and can be uniquely identified also in blood and lymphoid tissues by this phenotype.
Claims22
A method of enriching for CD11c− dendritic cells from a population of peripheral blood mononuclear cells, comprising:
- contacting said population with an anti-CD123 antibody;
- and then
- selecting the cells that are CD123+.
A method of distinguishing CD11c− dendritic cells in a population of peripheral blood mononuclear cells comprising:
- contacting said population with an anti-CD123 antibody, an anti-HLA-DR antibody, an anti-CD4 antibody and at least one antibody with specificity for a non-dendritic cell lineage marker;
- and
- detecting the cells that are CD123+HLA-DR+CD4+ and negative for expression of said non-dendritic cell lineage marker.
A method of distinguishing CD11c− dendritic cells within a population of peripheral blood dendritic cells, comprising:
- contacting said population with an anti-CD123 antibody;
- and then
- detecting the cells that are CD123+.
A method of depleting CD11c− dendritic cells from a population of peripheral blood mononuclear cells, comprising:
- contacting said population with an anti-CD123 antibody;
- and then
- removing the cells that are CD123+.
In a mixed lymphocyte reaction, the improvement comprising:
- incubating the lymphocytes in said reaction with dendritic cells enriched for CD123+CD11c− dendritic cells.
Dependencies are inferred from the claim text.