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US 6,294,372 B1Granted
Replication genes and gene products from small cryptic plasmids and methods for constructing controlled-replication plasmid vectors
AssigneeUniversity of Victoria Innovation & Dev. Corp.
InventorsJàn Burian, William W. Kay
Life of this patent
Estimating the term…
Abstract
The replication genes of small cryptic plasmids are isolated and used to construct controlled-replication plasmid vectors with the wide range of copy numbers controlled by defined helper plasmids. Controlled-replication vectors (RAMP vectors) can reach very high level of plasmid replication, which is not lethal to host unlike runaway replication vectors.
Claims11
An isolated nucleic acid molecule which encodes a repA protein, wherein said nucleic acid molecule is selected from the group consisting of:
- (a) a nucleic acid molecule which encodes a protein comprising the amino acid sequence of RepA wherein said protein is encoded by nucleotides 913 to 1386 of Sequence I.D. No:
- 19;
- (b) an isolated nucleic acid molecule that detectably hybridizes to the nucleic acid molecule of (a) or its complementary strand under conditions of 5×SSPE, 0.5% SDS, 1×Denhardt's, at 65° C. or equivalent salt and temperature conditions;
- (c) an isolated nucleic acid molecule that encodes a protein that has greater than 75% sequence homology to the protein encoded by the nucleic acid molecule according to (a);
- and
- (d) a nucleic acid molecule that, due to degeneracy of the genetic code, encodes a protein encoded by the nucleic acid molecule according to (b) or (c).
3 more elements
A vector designated pKL1.
Dependencies are inferred from the claim text.