US 6,294,358 B1 · Thermus promoters for gene expression

Abstract

The present invention relates to a system for identifying, isolating and utilizing promoter elements useful for expression of nucleotide sequences and the proteins encoded thereby in a thermophile. In one embodiment, a recombinant DNA molecule is provided, and comprises a reporter sequence, a putative thermophile promoter, a selectable marker sequence, and a 3′ and a 5′ DNA targeting sequence that are together capable of causing integration of at least a portion of said DNA molecule into the genome of a thermophile. Further, within the recombinant DNA, the reporter sequence is under the transcriptional control of a promoter which functions in a thermophile to form a promoter/reporter cassette, the promote/reporter cassette is flanked by said 3′ and said 5′ DNA targeting sequences, and the promoter/reporter cassette is positioned in the opposite orientation of the DNA targeting sequences.

Claims9

Dependent claims

A recombinant expression vector comprising a thermophile promoter, said promoter comprising a DNA sequence selected from the group consisting of SEQ ID NO:

5, SEQ ID NO:
6, SEQ ID NO:
7, SEQ ID NO:
8, SEQ ID NO:
9, SEQ ID NO:
10, SEQ ID NO:
12, SEQ ID NO:
24, SEQ ID NO:
25, SEQ ID NO:
26, SEQ ID NO:
27, SEQ ID NO:
28, SEQ ID NO:
29, SEQ ID NO:
30, SEQ ID NO:
31, SEQ ID NO:
32, SEQ ID NO:
33, SEQ ID NO:
34, SEQ ID NO:
35, SEQ ID NO:
36, SEQ ID NO:
37, SEQ ID NO:
38, SEQ ID NO:
39, SEQ ID NO:
40, SEQ ID NO:
41, SEQ ID NO:
42, SEQ ID NO:
43, and SEQ ID NO:
44.

25 more elements

Dependencies are inferred from the claim text.

Thermus promoters for gene expression

Life of this patent

Thermus promoters for gene expression

Life of this patent

Abstract

Claims9