US 6,294,345 B1 · Genes encoding proteins essential for plant growth and methods of use

Abstract

The present invention provides plant ENR-A, CBL, UROD, PBGD, and CPPO genes. Also disclosed are the recombinant production of ENR-A, CBL, UROD, PBGD, and CPPO enzymes in heterologous hosts, screening chemicals for herbicidal activity using these recombinantly produced enzymes, and the use of thereby identified herbicidal chemicals to suppress the growth of undesired vegetation. Furthermore, the present invention provides methods for the development of herbicide tolerance in plants, plant tissues, plant seeds, and plant cells using ENR-A, CBL, UROD, PBGD, and CPPO genes of the invention.

Claims8

Dependent claims

A method of identifying herbicidal compounds, comprising:

a) combining a plant polypeptide having porphobilinogen deaminase activity and comprising an amino acid sequence at least 90% identical to SEQ ID NO:8 with a compound to be tested for the ability to bind to said polypeptide, under conditions conducive to binding;
b) selecting a compound identified in step (a) that binds to said polypeptide;
c) applying a compound selected in step (b) to a plant to test for herbicidal activity;
and
d) selecting a compound identified in step (c) that has herbicide activity.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

09/586,719Filed 5 Jun 2000 · us provisional application

Priority

27 Jul 1999

Granted

25 Sep 2001

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12Q 1/527
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…
G01N 2430/20
Assays, e.g. immunoassays or enzyme assays, involving synthetic organic compounds as analytes: herbicides, e.g. DDT
G01N 2500/00
Screening for compounds of potential therapeutic value
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms

Family

Application60/198,218Us provisional application · filed 27 Jul 1999

Application60/240,929Us provisional application · filed 20 Aug 1999

Application60/228,810Us provisional application · filed 22 Nov 1999

Application60/287,572Us provisional application · filed 27 Mar 2000

Application60/287,571Us provisional application · filed 22 May 2000

US 6,294,345 B1This patent

From the US filing history. Foreign family members aren’t listed.

Genes encoding proteins essential for plant growth and methods of use

Life of this patent

Genes encoding proteins essential for plant growth and methods of use

Life of this patent

Abstract

Claims8