Genetic test for equine severe combined immunodeficiency disease
AssigneeBoard of Regents, The University of Texas System
InventorKatheryn D. Meek
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Abstract
The present invention relates to the discovery of the mutation of a DNA-dependent protein kinase protein which results in equine severe combined immunodeficiency (SCID). Specifically, the present invention provides the sequence of the normal and SCID DNA-dependent protein kinase genes, proteins, and provides diagnostic tests for identifying carriers of the mutation utilizing oligonucleotides that differentiate between the normal and the SCID alleles.
Claims14
An oligonucleotide of 15 to 25 bases that hybridizes to the severe combined immunodeficiency (SCID) determinant region of nucleic acid encoding the DNA-dependent protein kinasecatalytic subunit in Arabian horses (SEQ ID No:28).
A primer pair that amplifies the severe combined immunodeficiency (SCID) determinant region of nucleic acid encoding the DNA-dependent protein kinasecatalytic subunit in Arabian horses (SEQ ID No:28).
A method of identifying an Arabian horse that is a cater for equine severe combined immunodeficiency comprising determining the presence or absence of a gene encoding a functional DNA-dependent protein kinasecatalytic subunit in Arabian horses (SEQ ID No:28).
A method of identifying an Arabian horse that is a carrier of equine severe combined immunodeficiency comprising the step of determining whether said horse has a mutation in the severe combined immunodeficiency (SCID) determinant region of nucleic acid encoding the DNA-dependent protein kinasecatalytic subunit in Arabian horses (SEQ ID No:28).
A method of identifying an Arabian horse that is a cattier of equine severe combined immunodeficiency comprising the step of determining whether said horse carries the severe combined immunodeficiency (SCID) allele of the DNA-dependent protein kinasecatalytic subunit in Arabian horses, said allele comprising SEQ ID No:25.
An isolated DNA molecule encoding a DNA-dependent protein kinasecatalytic subunit of SEQ ID No:29.
Dependencies are inferred from the claim text.