Use of primers for universal fingerprint analysis
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorWolfgang Rohde
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Abstract
The invention relates to the use of primers or primer pairs for DNA fingerprint analysis, wherein with the primers or primer pairs fingerprints are obtainable from humans as well as from animals as well as from plants as well as from microorganisms. The invention further relates to primers or primer pairs for the above-mentioned use.
Claims36
A method for DNA fingerprint analysis consisting essentially of annealing a single primer consisting of 15-25 contiguous nucleotides of the complement of a 1.4 kilobasepair copia-like element of the DNA of Cocos nucifera L. to a sample of genomic DNA, obtaining a plurality of polymerase chain reaction products products by performing a polymerase chain reaction using said single primer and obtaining a DNA fingerprint by separating the polymerase chain reaction products.
A method for DNA fingerprint analysis comprising annealing at least one primer consisting of 15-25 contiguous nucleotides of the complement of a 1.4 kilobasepair copia-like element of the DNA of Cocos nucifera L. to a sample of genomic DNA, wherein said sample of genomic DNA is obtained from an organism other than a plant of the genus Cocos, obtaining a plurality of polymerase chain reaction products by performing a polymerase chain reaction and obtaining a DNA fingerprint by separating the polymerase chain reaction products.
A primer pair selected from the group consisting of SEQ ID NOS:5 and 10, SEQ ID NOS:
- 6 and 9, and SEQ ID NOS:6 and 10.
Dependencies are inferred from the claim text.