Analyte detection process using dual labeled probes

A method for detecting the presence of a target in a test sample comprising the steps of:

• (a) forming a reaction mixture comprising the test sample, a primer, a probe and amplification reagents wherein
• (i) the probe has a first and second label wherein the first label comprises a first specific binding member, and
• (ii) the primer and probe hybridize to the target such that the probe is downstream from the primer;
• (b) hybridizing the primer and probe to the target sequence and degrading the hybridized probe in a target dependent manner to separate the first and second labels;
• (c) contacting the reaction mixture with a capture reagent to form a first specific-binding-member/capture reagent complex;
• (d) detecting the second label associated with the first specific-binding-member/capture reagent complex, wherein a loss of signal indicates the presence of the target sequence;
• wherein said amplification reagents comprise an enzyme having 5′→3′ exonuclease activity capable of degrading the hybridized probe only when the probe is hybridized to the target sequence.