Cells for the production of recombinant adenoviruses
AssigneeRhone-Poulenc Rorer S.A.
InventorsEmmanuelle Vigne, Michel Perricaudet, Jean-Fran.cedilla.ois Dedieu, Cecile Orsini, Patrice Yeh, Martine Latta, Edouard Prost
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Abstract
The invention relates to cells usable for the production of defective adenoviruses comprising, inserted into their genome, a portion of the region E4 of an adenovirus genome carrying the reading phase ORF6 under the control of a functional promoter.
Claims33
A recombinant cell line for the production of a defective adenovirus comprising, inserted into its genome, part of an adenovirus E4 region comprising an ORF6 reading frame under the control of a functional promoter, wherein the inserted E4 region does not contain a functional ORF4 reading frame.
A plasmid comprising part of an E4 region of an adenovirus genome carrying a reading frame ORF6 under the control of an inducible promoter.
A defective recombinant adenovirus .DELTA.E1,ORF3.sup.+,ORF6.sup.-, wherein all or part of the E1 region and of nucleotides 34801-34329 and 34115-33126 of the Ad5 E4 region, or the corresponding nucleotides from Ad2, Ad7 or Ad12, are deleted.
A defective recombinant adenovirus .DELTA.E1,.DELTA.E4,ORF1.sup.+, wherein all or part of the E1 region and the E4 region with the exception of reading frame ORF1 are deleted.
A defective recombinant adenovirus .DELTA.E1,.DELTA.E4,ORF4.sup.+, wherein all or part of the E1 region and the E4 region with the exception of the reading frame ORF4 are deleted.
A defective recombinant adenovirus .DELTA.E1,.DELTA.E4, wherein all or part of the E1 region and the whole of the E4 region, chosen from the group consisting of Ad5 nucleotides 33466-35355 and 33093-35355, or the corresponding nucleotides from Ad2, Ad7 or Ad12, are deleted.
Dependencies are inferred from the claim text.