Borna disease viral sequences, diagnostics and therapeutics for nervous system diseases
AssigneeThe Regents of the University of California
InventorsW. Ian Lipkin, Thomas Briese, Stefanie Kliche, Patrick A. Schneider, Lothar Stitz, Anette Schneemann
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Abstract
The present invention presents: genomic nucleotide sequence of Borna disease virus, nucleotide and amino acid sequences of Borna disease virus proteins, recombinant viral proteins, vectors and cells containing the sequences or encoding the proteins, ligand binding to these proteins such as antibodies, and the diagnostic and therapeutic uses of the foregoing.
Claims18
A method of detecting, in a sample, a ligand capable of binding a BDV protein, said method comprising the steps of:
- a) contacting the sample with at least one isolated protein selected from the group consisting of:
- p23, recp23, and a protein comprising an amino acid sequence as set forth in SEQ ID NO:4, wherein said isolated protein is prebound to a solid support without gel size fractionation;
- and
- b) determining whether a ligand binds to the protein.
An immunoassay method of testing a sample for antibody ligands immunoreactive with Borna disease virus, comprising the steps of;
- a) contacting a test sample with an isolated protein, said protein being prebound to a solid support without gel size fractionation, wherein said prebound isolated protein is selected from the group consisting of:
- p23, recp23, and a protein comprising an amino acid sequence as set forth in SEQ ID NO:4;
- b) contacting the test sample with at least one other prebound isolated protein, wherein the other prebound isolated protein is selected from the group consisting of:
- gp18, recp18, p57, recp57, pol, recpol, p40, recp40, and a protein comprising an amino acid sequence as set forth in SEQ ID NO:
- 2, 6, 8, or 10;
- and
- c) detecting whether any antibody ligands in the sample bind to any of the prebound isolated proteins.
A method for selecting for a ligand capable of binding p23, said method comprising the steps of:
- a) contacting the ligand with an isolated protein prebound to a solid support, wherein the prebound isolated protein is selected from the group consisting of:
- p23, recp23, and a protein comprising an amino acid sequence as set forth in SEQ ID NO:4;
- and
- b) selecting for the ligand which binds to the protein.
Dependencies are inferred from the claim text.